nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression
نویسندگان
چکیده
objective(s): present study was performed in order to uncover new aspects for nicotine-induced damages on spermatogenesis cell lineage. materials and methods: for this purpose, 36 mature male wistar rats were divided into three groups as; control-sham (0.2 ml, saline normal, ip), low dose (0.2 mg/kg bw-1, ip) nicotine-received and high dose (0.4 mg/kg bw-1, ip) nicotine-received groups. following 7 weeks, the expression of bcl-2, p53 and caspase-3 at mrna and protein levels were investigated by using reverse-transcriptase pcr (rt-pcr) and immunohistochemical (ihc) analyses, respectively. moreover, the serum level of fsh, lh and testosterone were evaluated. finally, the mrna damage was analyzed by using special fluorescent staining. results: nicotine, at both dose levels, decreased tubular differentiation, spermiogenesis and repopulation indices and enhanced cellular depletion. animals in nicotine-received groups exhibited a significant (p<0.05) reduction at mrna and protein levels of bcl-2. more analyses revealed a remarkable (p<0.05) enhancement in expression of p53 and caspase-3 in comparison to control-sham animals. finally, nicotine resulted in a significant (p<0.05) reduction in serum level of testosterone and elevated mrna damage. conclusion: our data showed that, nicotine by suppressing the testosterone biosynthesis, reducing mrna and protein levels of bcl-2 and up regulating the p53 and caspase-3 mrna and protein levels adversely affects the spermatogenesis and results in cellular depletion.
منابع مشابه
Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression
Objective(s): Present study was performed in order to uncover new aspects for nicotine-induced damages on spermatogenesis cell lineage. Materials and Methods: For this purpose, 36 mature male Wistar rats were divided into three groups as; control-sham (0.2 ml, saline normal, IP), low dose (0.2 mg/kg BW-1, IP) nicotine-received and high dose (0.4 mg/kg BW-1, IP) nicotine-received groups. Followi...
متن کاملNicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression
OBJECTIVES Present study was performed in order to uncover new aspects for nicotine-induced damages on spermatogenesis cell lineage. MATERIALS AND METHODS For this purpose, 36 mature male Wistar rats were divided into three groups as; control-sham (0.2 ml, saline normal, IP), low dose (0.2 mg/kg BW-1, IP) nicotine-received and high dose (0.4 mg/kg BW-1, IP) nicotine-received groups. Following...
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عنوان ژورنال:
iranian journal of basic medical sciencesجلد ۲۰، شماره ۲، صفحات ۱۹۹-۲۰۸
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